Mouse Anti-HSPA1A Recombinant Antibody (HPAB-0243-YC)
CAT#: HPAB-0243-YC
The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Overexpressed inside cells, Hsp70 is transported to the cell membrane and also exported into the extracellular space. Provided herein are anti-Hsp70 monoclonal antibodies, which redirect immune cell specificity towards Hsp70 positive cells.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Recombinant Mouse Anti-HSPA1A Antibody (HPAB-0243-YC) in SDS-PAGE
Lane M: Marker
Lane 1: HPAB-0243-YC in non-reduced condition.
Lane 2: HPAB-0243-YC in reduced condition.
HPLC
Figure 2 Recombinant Mouse Anti-HSPA1A Antibody (HPAB-0243-YC) in HPLC
The purity of HPAB-0243-YC was greater than 92% as determined by SEC-HPLC.
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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in the basal layer of glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_1_5_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enriched (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204389-HSPA1A
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPA1A
- Species Reactivity
- Human
- Applications
- ELISA, FC
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Flow Cytometry. For details, refer to Published Data.
Target
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(Creative Biolabs Cat# HPAB-0243-YC, RRID: AB_3111375)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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